In 2018, CDC's Division of TB Elimination (DTBE) began universal whole-genome sequencing (WGS) (i.e., sequencing an M. tuberculosis isolate for each U.S. case of culture-confirmed TB). Increasingly, state and local programs are using results of WGS and phylogenetic analyses to help guide TB cluster and outbreak investigations. A training module on "Whole-genome sequencing for investigation of recent TB transmission in the United States: Current uses and future plans" is available here. Comments on this video are allowed in accordance with our comment policy: http://www.cdc.gov/SocialMedia/Tools/CommentPolicy.html This video can also be viewed at https://www.cdc.gov/tb/programs/genotyping/video/TuberculosisWGS-TrainingModules-2_LowerRes.mp4